Concentration Of The Sucrose Solution Biology Essay

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All the method is referred to the sheet given. In order to dilute1.0 mol dm-3 of sucrose solution given to produce 0.2 mol dm-3 of sucrose solution, 0.3 mol dm-3 of sucrose solution, 0.4 mol dm-3 of sucrose solution and 0.5 mol dm-3 of sucrose solution, the formula below is used ;

M1V1 = M2V2

For example:

To produce 0.2 mol dm-3 of sucrose solution with volume of 20cm3

M1 (initial concentration of sucrose solution) = 1.0 mol dm-3

V1 (initial volume of sucrose solution) = ?

M2 (final concentration of sucrose solution) = 0.2 mol dm-3

V2 (initial volume of sucrose solution) = 20 cm3

M1V1 = M2V2

mol dm-3)V1 = (0.2 mol dm-3)(20 cm3)

V1 = (0.2 mol dm-3)(20 cm3)

(1.0 mol dm-3)

V1 = 4 cm3

RESEARCH QUESTIONS

Does verifying the concentration of sucrose solution would affect the length of potato strips and apple strips after 125 minutes?

Does the volume of the sucrose solution affected?

HYPOTHESIS

If the concentration of the sucrose solution is high, which is a hypertonic concentration, the length of potato strips and apple strips will decrease because the water molecules moved out from the potato and apple tissue to the sucrose solution. This causes a decrease in the length of potato and apple strips. Thus, the potato and the apple strips become flaccid. The volume of the sucrose solution will increase simultaneous due to the decreased of potato and apple lengths.

If the concentration of the sucrose solution is low, which is a hypotonic concentration, the length of potato and apple strips size will increase and becomes turgid because the water moved out of the potato and the apple cells. The volume of the sucrose solution will decrease simultaneously with the decreased of potato and apple lengths.

If the concentration of the sucrose solution is same as the concentration of potato and apple tissues, there will be no change on the length of potato and apple strips because the movement of water molecules into and out of the potato and apple cells is the same. The volume of the sucrose solution also will not changed. This called isotonic.

VARIABLES :

Independent variable :

Independent variable

Concentration of sucrose

Dependent variable

Final length of potato and apple strips after 125 minutes

Constant variable

Initial length of apple and potato strips

Initial volume of sucrose solution

Five strips of apple and potato are put in each test tube

Raw Data

Concentration of sucrose solution (mol dm-3)

Volume of 1.0 mol dm-3 of sucrose solution (cm3)

Volume of distilled water (cm3)

0.2

4

16

0.3

6

14

0.4

8

12

0.5

10

10

1.0

20

0QUALITATIVE DATA

Table 1: The volume of sucrose solution and volume of distilled water needed

to dilute the 1.0 mol dm-3 of sucrose solution.

Observation on the changes occurs to the apple strips and potato strips.

molarities of sucrose solution (mol dm-3)

0.2

0.3

0.4

0.5

1.0

Change in length

longer

longer

Same

shorter

shorter

Change in level of

sucrose solution

decrease

decrease

same

increase

increase

Texture and appearance

turgid

less turgid

firm

soft

soft

Table 2: Changes occur to apple strips after the experiment

molarities of sucrose solution (mol dm-3)

0.2

0.3

0.4

0.5

1.0

Change in length

longer

longer

same

same

shorter

Change in level of

sucrose solution

decrease

decrease

decrease

decrease

increase

Texture and appearance

crunchy

crunchy

crunchy

crunchy

soft

Table 3: Changes occur to potato strips after the experiment

QUANTITATIVE DATA

length of apple strips, cm (±0.05cm)

molarity of sucrose solution

strip 1

strip 2

strip 3

strip 4

strip 5

(mol dm-3)

initial

final

initial

final

initial

final

initial

final

initial

final

0.2

4.0

4.1

4.0

4.2

4.0

4.1

4.0

4.0

4.0

4.2

0.3

4.0

4.3

4.0

4.1

4.0

4.1

4.0

4.2

4.0

4.1

0.4

4.0

3.9

4.0

4.0

4.0

4.1

4.0

4.0

4.0

3.9

0.5

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

1.0

4.0

3.7

4.0

3.7

4.0

3.8

4.0

3.8

4.0

3.8

Table 4a: The length of 5 apple strips before and after 120 minutes.

molarity of sucrose solution

(mol dm-3)

Initial volume of sucrose solution,

(± 0.05cm3)

Final volume of sucrose solution,

(± 0.05cm3)

0.2

20.00

18.00

0.3

20.00

17.00

0.4

20.00

16.50

0.5

20.00

18.00

1.0

20.00

20.50

Table 4b: The initial and final volume of sucrose solution for apple strips

length of potato strips, (±0.05cm)

molarity of sucrose solution

strip 1

strip 2

strip 3

strip 4

strip 5

(mol dm-3)

initial

final

initial

final

initial

final

initial

final

initial

final

0.2

4.0

4.4

4.0

4.5

4.0

4.2

4.0

4.1

4.0

4.1

0.3

4.0

4.0

4.0

4.1

4.0

4.1

4.0

4.1

4.0

4.1

0.4

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

0.5

4.0

3.8

4.0

3.8

4.0

3.9

4.0

3.7

4.0

3.7

1.0

4.0

3.5

4.0

3.5

4.0

3.6

4.0

3.7

4.0

3.4Table 5a: The length of 5 potato strips before and after 120 minutes

molarity of sucrose solution

(mol dm-3)

Initial volume of sucrose solution,

(± 0.05cm3)

Final volume of sucrose solution,

(± 0.05cm3)

0.2

20.00

19.00

0.3

20.00

20.00

0.4

20.00

20.00

0.5

20.00

21.00

1.0

20.00

21.00

Table 5b: The initial and final volume of sucrose solution for potato strips

DATA PROCESSING

Average = length of (strip 1 + strip 2 + strip 3 + strip 4 + strip 5)

5

length of apple strips, (±0.05cm)

molarity of sucrose solution

strip 1

strip 2

strip 3

strip 4

strip 5

Average

(mol dm-3)

initial

final

initial

final

initial

final

initial

final

initial

final

initial

final

0.2

4.0

4.1

4.0

4.2

4.0

4.1

4.0

4.0

4.0

4.2

4.0

4.1

0.3

4.0

4.3

4.0

4.1

4.0

4.1

4.0

4.2

4.0

4.1

4.0

4.2

0.4

4.0

3.9

4.0

4.0

4.0

4.1

4.0

4.0

4.0

3.9

4.0

4.0

0.5

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

1

4.0

3.7

4.0

3.7

4.0

3.8

4.0

3.8

4.0

3.8

4.0

3.8

Table 6a: Average length of apple strips

Difference of apple strips length = Final reading - initial reading

molarity of sucrose solution

(mol dm-3)

Initial reading, (±0.05cm)

Final reading, (±0.05cm)

Difference in reading (±0.1cm)

0.2

4.0

4.1

0.1

0.3

4.0

4.2

0.2

0.4

4.0

4.0

0.0

0.5

4.0

4.0

0.0

1.0

4.0

3.8

-0.2

Table 6b: Difference in reading of the apple strips

Average = length of (strip 1 + strip 2 + strip 3 + strip 4 + strip 5)

5

length of potato strips, (±0.05cm)

molarity of sucrose solution

strip 1

strip 2

strip 3

strip 4

strip 5

Average

(mol dm-3)

initial

final

initial

final

initial

final

initial

final

initial

final

initial

final

0.2

4.0

4.4

4.0

4.5

4.0

4.2

4.0

4.1

4.0

4.1

4.0

4.3

0.3

4.0

4.0

4.0

4.1

4.0

4.1

4.0

4.1

4.0

4.1

4.0

4.2

0.4

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

4.0

0.5

4.0

3.8

4.0

3.8

4.0

3.9

4.0

3.7

4.0

3.7

4.0

3.8

1

4.0

3.5

4.0

3.5

4.0

3.6

4.0

3.7

4.0

3.4

4.0

3.5

Table 7: Average length of potato strips

Difference of potato strips length = Final reading - initial reading

molarity of sucrose solution

(mol dm-3)

Initial reading, (±0.05cm)

Final reading, (±0.05cm)

Difference in reading (±0.1cm)

0.2

4.0

4.3

0.3

0.3

4.0

4.2

0.2

0.4

4.0

4.0

0.0

0.5

4.0

3.8

-0.2

1.0

4.0

3.5

-0.5

Table 7b: Difference in reading of the potato strips

CONCLUSION

If the concentration of the sucrose solution is high, which is a hypertonic concentration, the length of potato strips and apple strips will decrease because the water molecules moved out from the potato and apple tissue to the sucrose solution. This causes a decrease in the length of potato and apple strips. Thus, the potato and the apple strips become flaccid. The volume of the sucrose solution will increase simultaneously with the decreased of potato and apple lengths. The hypertonic solution for apple strips is at 0.2M and 0.3M, whereas for the potato strips is at 0.2M and O.3M also.

If the concentration of the sucrose solution is low, which is a hypotonic concentration, the length of potato and apple strips size will increase and becomes turgid because the water moved out of the potato and the apple cells. The volume of the sucrose solution will decrease simultaneously with the decreased of potato and apple lengths. The hypotonic solution for apple strips is at 0.5M and 1.0M, whereas for the potato strips is at 1.0M.

If the concentration of the sucrose solution is same as the concentration of potato and apple tissues, there will be no change on the length of potato and apple strips because the movement of water molecules into and out of the potato and apple cells is the same. The volume of the sucrose solution also will not changed. This called isotonic. The isotonic solution for the apple strips is at 0.4M, whereas for the potato strips is 0.4M and 1.0M.

The hypothesis is accepted.

LIMITATIONS

SUGGESTIONS

Error may occur during the measurement to determine the length of potato strips.

the measurement should be repeated for at least 3 times to minimize the error in the experiment.

Error may occur while marking the level of sucrose solution due to the poor method of measuring that is by visual observation.

A more precise tool should be used to get the most precise data.

. The surface of potato strips may not flat. So even though we controlled the initial length,actually the initial length are not same for each potato strips

the mass of potato strips in order to observe the changes of elongation of the potato strips in each solution to get more accurate reading as the electronic mass balance has a small uncertainty compared than using a ruler to measured the elongation.LIMITATIONS AND SUGGESTIONS

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