How Glucose Concentration Affects Mass Biology Essay

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I was shown an example of Osmosis, which involved a potato chip and 5% glucose solution; we saw that as the glucose solution, was left with the potato given 24 hours, the potato had gained mass due to the glucose solution being more concentrated than the potato chip. Here is a scientifically correct definition of Osmosis.

I set up 5 flasks for this experiment, and added in each beaker 30ml of one the four different solutions and then distilled water (tap), I then labelled accordingly. I then weighed each potato chip and tried to get them more or less 0.9g and then measured them for added accuracy. I also recorded the texture before and after the test. I then left the preliminary test beakers until the end of the lesson where I recorded the results and analyses them next lesson. What I concluded from the results was that the potato chip in the distilled water, gained the most mass. This happened because the potato is semi-permeable and the water molecules were small enough to fit in and the water goes to the place where there is less concentration. It took longer for the water to get into the potato from the sucrose solution, because there were fewer molecules on the outside. From preliminary testing I decided on a suitable range for the real test of about 0.10-0.40 change in mass.

I have identified a possible factor which may affect the outcome of my investigation which is Temperature. An increase in temperature acts as a catalyst which speeds up reactions, although osmosis isn't a reaction I have had to think about the effect of which it could have, and I have concluded from speaking with colleagues and professionals that it would affect osmosis. We know that osmosis is caused by molecule movements so, when temperature goes higher, molecules move faster. So, Osmosis will increase.

If we decrease temperature, the rate of Osmosis will decrease too. I have taken control of this by ensuring that all the beakers remain the same temperature, so by leaving them at room temperature in the same room this should eliminate temperature from affecting my results.

Modifications to initial plan from preliminary test

I decided to increase the amount of solution to guarantee a sustained period of osmosis, and the starting mass of the potato chip to increase it from 0.6g to 0.9g to enable more clear results and larger numbers to deal with and also to leave the test for longer (approx 24 hours) to ensure that osmosis has taken place and given more of a chance to act and for us to notice results. From the preliminary test some equipment I have decided to change where appropriate for valid reasons. I have decided to use the smaller measuring cylinder to measure the solution more accurately.

Equipment

Quantity

Use

Beaker

5

To hold solution and potato chip for 24 hours.

Potato Chips

8

Semi-Permeable Membrane, (Extra in case of breakages).

Solutions (1%,2%,5%,10% & 0%).

40 ml of each

It is the basis of the investigation.

Ruler

1

To measure length of potato chips.

Measuring Cylinder ( Small)

1

To measure the solution accurately.

Board & Scalpel

1

To cut up potato chips.

I have chosen to use beakers not conical flasks as getting the potato chips out of the flash without causing breakages at the end would be nigh on impossible and then to accurately get results like length could easily be affected. I chose to use 40ml of solution as it covers the potato chips fully and is a reasonable amount without extra unnecessary solution.

I thought it would be a good idea on how to better evaluate my test like showing a step by step plan on my procedure and provide justification of doing so.

Label 5 Beakers with solution names and my initials (for easy recognition).

Measure and add 40ml of the correct solution into each beaker.

Then measure and weigh potato chips, weight must be within 0.05g of 0.9g

Take note of solution chips added to, and then leave for approx 24 hours.

Real Test

The next stage of the investigation was to complete the actual test, using the above plan and modifications here are the results below. I needed to ensure this was going to stay a fair test so I needed to accurately measure the solution and potato chips each time, as if not done properly or written down incorrectly it could affect my results. I also needed to place the chips in at the same time. There was only one variable which was the solution and everything else had to stay the same, with one exception of the potato chips with a 0.05g leeway.

Solution

Mass

Length

Texture After

Change in Mass

-

Before

After

Before

After

-

-

Distilled Water

0.95

1.26

30mm

35mm

Hard

0.31

1%

0.93

1.21

30mm

34mm

Hard

0.28

2%

0.94

1.15

30mm

33mm

Hard

0.21

5%

0.93

1.12

30mm

32mm

Hard

0.19

10%*

Anomaly* 

0.87

0.87

1.01

1.12

30mm

30mm

32mm

32mm

Hard

Hard

0.14

0.25

By looking at the results I can see a very clear trend. With that the higher the concentration the lower the change in mass and there is also a similar pattern in length with the most growth in the less concentrated solutions. This is an inverse relationship.

I have identified an anomaly from my data; I decided this was an anomaly as it didn't fit the general trend of the experiment and goes against my osmosis theory. I concluded this wouldn't be fair to base a graph on it, or use it in my results so I repeated the test for the 10% solution.

I felt I needed to get another set of results so I could write a comparison of sort, so because we didn't have enough time in class to repeat the experiment, I borrowed a reliable set of results from a friend. By having 2 sets of data, I can either use the second set to back-up the reliability of my data or to scrutinise it.

Solution

Mass

Length

-

Before

After

Before

After

Water

0.63g

0.88g

18mm

Permission to use their data was kindly granted by Laura Barker.22mm

1%

0.60g

0.77g

19mm

21mm

2%

0.56g

0.68g

18mm

20mm

5%

0.58g

0.67g

18mm

20mm

10%

0.58g

0.58g

18mm

18mm

In comparison the results differ but both show similar trends for Mass and Length. The changes are different, but this is most probably due to the different constants that they used which was 20ml of solution not 40ml.

I have produced an appropriate scatter graph which shows a correlation between the concentrations of solution with the change in mass. I only chose to show the change in mass, as I felt that this showed the most significant change to base my conclusion on. The graph shows a definitive strong negative correlation between mass and concentration of solution, which is what I expected in relation to my hypothesis. The graph shows that the concentration within the cell is 10% or higher, we could predict this using the lines of best fit, and their equations.

I observed the potato chips and noticed that the skin of the potato stretched which provided again more evidence of the potatoes dilation. I also observed that

Conclusion

In Conclusion I believe that Osmosis has happened within the cell (potato chip) due to valuable evidence by 2 sets of data. Although I cannot work out averages for this particular experiment as we didn't have time to repeat there can be some limitations. But we can guess that the potato chip has a higher concentration of glucose in its cell, as it expanded and gained molecules, where it obviously didn't lose any because all chips gained mass. So we can see that 10% is probably a similar percentage of concentration of that in the potato cell. I also notice an inverse relationship by looking at my graphs and the numerical data; I can see that when the solution strength increases the change in mass decreases, this proves the theory of osmosis. The range of my experiment was sufficient enough to prove the theory of Osmosis.

Evaluation

I believe there could be alternative ways to collect the data I collected by maybe looking at a smaller mass of potato chip and only giving it a few hours for osmosis to happen.

There are also alternative methods to prove osmosis. Instead of changing the solution strength I could have changed mass of the potato, surface area of the potato, use a more varied range of concentrations.

There have been limitations to my conclusion due to the fact I didn't have time to repeat the experiment. Possibly I could have placed two potato chips in each solution to save time, and maybe dye the second one a different colour possible using red cabbage juice or food colouring. I could have improves the accuracy of my data by perhaps measuring the girth of the potato chip before and after as well as the length.

The evidence was gathered fairly, with the chips being in the solution the same amount of time, time same amount of solution, and accurate readings, weighing & use of sophisticated equipment including eyesight, so I would believe the data is pretty reliable. We did have one anomaly but the test was repeated and a new result taken. My results relate to the theory of osmosis which therefore proves the reliability as being good.

To help make the data more secure we should have taken measurement of maybe the density of the potato chips or volume of it to help picture the change in mass. But the all results were precisely measured so the data should be reliable & secure. If I was to repeat the experiment I would have a larger range of concentration to pin point exactly the concentration of the potato as an added bonus.

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