Discuss the concept of recombinant Dna technology

Published:

This essay has been submitted by a student. This is not an example of the work written by our professional essay writers.

Before i get into gene therapy and genetic engineering i feel it is essential to discuss the concept of recombinant Dna technology; the technology that makes gene therapy possible. The procedure is to extract and cut up DNA from a donor genome into fragments and insert the needed fragments (fragments containing desired genes) into autonomously replicating DNA molecules such as bacterial plasmids. The plasmids act as vectors, or carriers for the inserted gene. Both the vector DNA and the Donor DNA are digested with the use of a restriction enzyme(same enzyme for each). Restriction enzymes are endonucleases, enzymes that cleave the phosphodiester bond in within a polynucleotide chain. Digestion of the plasmids with the restriction enzyme converts the circular plasmid DNA into a linear molecule with sticky ends. Each DNA sequence is then mixed in a test tube to allow the sticky ends of vector and donor DNA to bind together and form what are called recombinant molecules. The spliced DNA recombinants can be sealed back into plasmids by the addition of the enzyme DNA ligase,which binds the sugar phosphate backbone of DNA back together. The recombinant plasmid DNA is then taken up by bacterial cells, and these cells in turn divide into millions of other bacterial cells creating many clones of that gene. These transgenic bacterial cells are then able produce the proteins that correspond to the cloned gene.(source)

Gene therapy uses Recombinant Dna technology to replace a mutant allele with a funtioning one within the human genome to correct a genetic disorder.This acts genotypically and theoretically allows us to permanently cure a disease through a single therapeutic application. When the gene is replaced with a functional one the patients body should theoretically be able to produce its corresponding protein, thereby correcting the disorder. The process of gene therapy can be divided into four steps.

First, the wildtype gene of interest is isolated and cloned. Second, the DNA for the desired functional gene is inserted into an appropriate vector.This is accomplished using restriction enzymes to produce a gene/vector hybrid. A vector is a DNA carrier that is used to deliver the gene to the patients genome.Commonly used vectors are viruses that are modified to be harmless to humans. These vectors include modified retroviruses, adenoviruses etc. Other vectors have been used but the idea is to exploit the natural ability of a virus to seek out certain cells and insert their genes. One of the limitations of this method is that every current vector has at least some problems, and may be the reason for unsuccessful gene therapy trials (Marshall 2000a). The third step is a process called transfection where the gene/vector hybrid is inserted into the new host. This can be accomplished in two ways "In vivo" or "ex vivo." In vivo or "in the living tissue" involves injection of our vector hybrid directly into the patient's body where needed. Alternatively, ex vivo or "outside the living body" means the removal of human cells from the host, transfection of those cells and then reinsertion back into the host (Crystal 1995). This could be done on the host adult somatic cells and the results would only be temporary until the cell dies or it could be done to embryonic cells and the genes would be distributed throughout the entire body of the adult. If done on germ cells, the host would pass the gene on to subsequent generations. In the last step, the DNA must be integrated successfully by the host, transcribed and translated so that the host produces the new gene's product protein. This last step, however, involves issues with gene regulation, differentiation of cell types, and developmental stages. Because of this the inserted genes only expresses their proteins for a matter of weeks and then cease to function.(Human Gene Therapy)

History

Initially hypothesized as gene surgery, gene thearapy was proposed in 1983 for the treatment of inherited diseases caused by defective genes. It had been suggested and hypothesized since 1970 when the first restriction endonucleases (restriction enzymes) were isolated and this led to the onset of recombinant DNA technology. This new found technology allowed agricultural genetic engineering and transgenic animals, that in turn led to many medical advancements. However human genetic engineering, or what has now come to be known as gene therapy was not attemted till the 1990's. In 1990,The first human trial of gene therapy ever attempted was on a four-year-old girl. She was treated for severe combined immunodeficiency syndrome (SCID), a fatal inherited disease that that prevented her body from producing a crucial enzyme called adenosine deanimase(ADA). Researchers took the girl's white blood cells which were deficeient in ADA and infused them with ADA producing genes. These modified white blood cells were then transfered back to the patient. The yound girl continued to show increased stability but debate arose over whether this was the result of the gene theapy or the other drug treatments she had been receiving. She is alive today and is cured from the more adverse effecst of her illness but requires periodic conventional treatment to stay healthy..(Human Gene THERAPY)

Gene therapy trials continued on several inherited diseases including Familial hypercholesterolemia (FH) and Cystic fibrosis (CF). Treatment of familial hypercholesterolemia, an autosomal dominant mutation that causes elevated blood cholesterol levels which leads to heart attacks at a very young age, promised some degree of success but variation in patient's responses to the treatment precluded continuation of trials. Reasearch continues on Cystic fibrosis, though, a recessive mutation on chromosome 7 in the gene for cystic fibrosis transmembrane conductance regulator(CFTR). Defective proteins from this mutation results in the accumulation of mucus around the epitheliatl tissues of the lungs. THis environment is ideal for infectious germs that lead to chronic lung disease an early death (Zabner et al. 1993). Cystic Fibrosis can only be treated symptomatically and is the most common fatal genetic disorder among Caucasians. This makes it one of the most researched human diseases in gene therapy. There have been over a dozen trials, using a variety of vectors, in an attempt to deliver a wildtype CFTR gene to respiratory tract tissues but there have been limited results. Research continues as they attempt to find a cure for this genetic disaster.(Human Gene THERAPY)

On September 17, 1999, for the first time a patient died during human gene therapy trial. An 18-year-old male volunteer, died while being treated for partial ornithine transcarbamylase deficiency, a condition caused by a missing or defective X-linked gene responsible for producing the liver enzyme ornithine transcarbamylase (OTC) (Marshall 2000a).

A deficiency of this enzyme means the inability to break down nitrogenous compounds. This results in a toxic buildup of ammonia in the bloodstream and could be fatal. The young male however had a less severe case of the disease and was reasonably healthy before the trial.

The OTC was administered in an adenovirus chimera to the livers of 18 volunteers through their hepatic arteries. The 18 -year- old died four days after transfection due to an immune response to the adenovirus vector. In other words, The boy was killed by the vector and ultimately gene therapy. This revelation caused a huge setback for researchers and was a great impedement for the advancment of human getetic engineering as the FDA closed down all researsh in the field.

Sever Combine Immunodeficiency Syndrome comes in several forms. The first trials in of gene therapy in 1990 tried an autosomal disease. SCID- XIis, a more lethal form of SCID, is caused by an X-linked mutation in a receptor for cytokines, hormones that signal inflammatory response in cells. People who suffer from this disease have severe immune system failures that force them into quarentine as they cannot handle being exposed to even the slightest infection without it being fatal.(Marshall 2000a).

In may 2000 two infant girls, aged 8 and 11 months, with SCID-X1, underwent treatment by gene therapy. They extracted each patients bone marrow cells and incubated them with a Maloney- derived retrtheovirus vector carrying the cytokine receptor cDNA. After incubation was complete specialists inserted the modified cells back into the bone marrow. Ten months following this transfection, the two girls showed enough improvement in their immune systems that they could be taken out of protective isolation and moved home (Cavazzana-Calvo et al. 2000). This was the first known documentable success of gene therapy as it has until now cured a genetic condition. Trials have been ongoing since 1999, in the U.S. designed to treat 18 inherited diseases (Morgan & Blaese 1999).

Writing Services

Essay Writing
Service

Find out how the very best essay writing service can help you accomplish more and achieve higher marks today.

Assignment Writing Service

From complicated assignments to tricky tasks, our experts can tackle virtually any question thrown at them.

Dissertation Writing Service

A dissertation (also known as a thesis or research project) is probably the most important piece of work for any student! From full dissertations to individual chapters, we’re on hand to support you.

Coursework Writing Service

Our expert qualified writers can help you get your coursework right first time, every time.

Dissertation Proposal Service

The first step to completing a dissertation is to create a proposal that talks about what you wish to do. Our experts can design suitable methodologies - perfect to help you get started with a dissertation.

Report Writing
Service

Reports for any audience. Perfectly structured, professionally written, and tailored to suit your exact requirements.

Essay Skeleton Answer Service

If you’re just looking for some help to get started on an essay, our outline service provides you with a perfect essay plan.

Marking & Proofreading Service

Not sure if your work is hitting the mark? Struggling to get feedback from your lecturer? Our premium marking service was created just for you - get the feedback you deserve now.

Exam Revision
Service

Exams can be one of the most stressful experiences you’ll ever have! Revision is key, and we’re here to help. With custom created revision notes and exam answers, you’ll never feel underprepared again.