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Research question- How do the different concentrations of potato extract affect the enzyme activity?
- Hypothesis- If the concentration of the potato extract is higher, the time taken for the filter paper to float of the hydrogen peroxide solution, will be higher. This is because, at low potato extract concentration, the rate of enzyme activity, there are less collisions between the substrate and the active site where as if the potato extract concentration is higher, there are more collisions between the active site and the substrate.
- Variables- Independent- the independent variable for this experiment is the concentration of the potato extract which will be varied by changing the volume of the potato extract by adding different volumes of distilled water and decreasing the volume of the potato extract in each reading.
- Controlled variable- the controlled variable for this experiment is the concentration of the Hydrogen Peroxide (H2O2) in which is 10% and he volume taken for each reading is also kept the same in which is 5cm3. The total volume, of the potato extract and the distilled water will be maintained at 10 cm 3.
- Dependent variable- The dependent variable for this experiment is the time taken for the piece of filter paper to rise to the top of the hydrogen peroxide solution in the beaker.
- 3 Syringes of a total volume of 10 cm 3 each.
- Test tube
- Potato extract of a volume of 150cm3 and a concentration of 100%
- Distilled water of a volume of 150cm3
- Beakers (250 cm3, 50 cm3 )
- Filter paper cut into very small pieces.
- Hydrogen Peroxide(H2O2) of a volume of 150 cm3 and a concentration of 10%.
- Pour the potato extract in a beaker for all the trials.
- Pour distilled water into another beaker for all the trials.
- Pour hydrogen peroxide into another beaker for all the trials.
- Take one syringe, and measure 10 cm3 of potato extract and squirt it into the small beaker. Take another small beaker and use the other syringe to take 10 cm3 of H2O2 and pour it into another small beaker.
- Take the tweezer to pick up the minute piece of paper and dip it into the 100% concentrated potato extract until its wet enough.
- Drop it in into the hydrogen peroxide solution and start the stopwatch. The stop watch should be stopped when the piece of filter paper floats.
- Throw the potato extract and the hydrogen peroxide solution and wash the beakers
- Record the time taken
- Since the total volume of the concentrated potato extract and the diluted potato extract has to be 10 ml, repeat steps 5 to 8 for the concentrations of 75%, 50%, 30%,25%, and 10% of the extract.
- Steps 1 to 8 should be performed for the 2nd and the 3rd.
Results of the experiment:
My hypothesis was correct since my results show that because if the concentration is higher, the time taken for the piece of filter paper to float will be less. And if the concentration decreases, the time taken increases it is shown in the curve on the graph since the volume is decreasing the time taken is increasing.
There might have been some errors which could have caused minor fluctuations in the readings. The syringe used to take the potato extract out of the beaker, is difficult to take the readings so there might have been a difference in (+0.1cm3) in the volume used. This might have resulted in the change in the concentration of the potato extract causing the time taken for the filter paper to float to also fluctuate by (+1 sec).
The flaws in the procedure, could have been due to the usage of the syringe in which was in measuring the amount of the potato extract and the distilled water. There were no other errors which have caused a faulty reading.
All of the other important variables were controlled to avoid errors as much as possible as it has been shown in the proceedure.
took enough readings for the average to get the best possible results so there was no lack in the replication.
Improvements to be made:
Some improvements can be made by washing the tools used more often. More replications of the results should have been taken to plot a better curve on the graph. The readings on the syringe should have been taken more precisely.