# Aerobic Cellular Respiration Release Energy From Organic Compounds Biology Essay

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Aerobic cellular respiration is the release of energy from organic compounds by metabolic chemical oxidation in the mitochondria. (1) The equation c shows the complete oxidation of glucose. There are three ways to measure cellular respiration using this equation. How many moles are consumed in cellular respiration, moles of are produced in cellular respiration, and how much energy during cellular. In the cell respiration lab cellular respiration will be measured by determining the number of moles of consumed. The equation where P=pressure of gas, V=volume of gas, n=number of molecules of gas, R=gas constant, and T=temperature of the gas, is essential to understanding the four concepts of gasses that apply to this experiment. One is if the T and P are constant then the V and n are directly proportional. Another is if the T and V are constant then P changes indirectly proportion to n. The next is if n and T are constant then P is inversely proportional to the V. The last is if T changes and n is constant, then P or V or both will change directly proportional to T. the equation +2KOH means will be removed by KOH and will form , this occurs in the cellular respiration lab. In the experiment when the is removed the change in V will be directly related to the amount of oxygen consumed in the lab if the water T and V remain constant, water will move to the lower area of lower pressure. During the cellular respiration oxygen will be reduced. The respirometer will have a decrease in V and a decrease in P in the respirometer, as a net result in the glass beads respirometer any detection of changes in V caused by atmospheric P or T changes.

The purpose of the cellular respiration lab was to be able to calculate the rate of cell respiration from the experiments data, and to show how gas production relates to respiration rate within the experiment by using the differentiation in the water height in a pipette. Also being able to use the temperature baths to show how the rate of cell respiration in germinating vs. nongerminating seeds in a controlled experiment.

My hypothesis is since the T and V remain constant then the P and n will change in direction to each other. Also since in the equation +2KOH the T and V remain constant the water will move toward the end of lower pressure the water will be at the end next to the beads and beads. And the germinating peas will soak up the most water so they will put more oxygen.

## Materials/methods

Thermometer

2 baths( large trays)

A bucket of ice

Water to fill the baths

50 germinating peas

Paper towels

6 respirometers ( with a stopper and a pipette for each respirometer)

50 dried(nongerminating) peas

Enough peas to be added to the dried peas to make the volume of the germinating peas(10mL)

Absorbent cotton(the cotton balls)

Tape

15% KOH

Dropper

Obtained 2 bathes one was room temperature and the other was 10&deg;c. Adding ice ever so often made the second bath 10&deg;c.

100mL graduated cylinder and was filled with 25mL of O and 25 germinating peas were placed in the cylinder and the amount of water that was displaced after the peas were put in was the volume of the germinating peas. The peas were taken out of the cylinder and placed on a paper towel to be saved for respirometer 1

The graduated cylinder was refilled with 25mL of O. 25 non germinating peas were placed in the cylinder the glass beads were added to the cylinder to make the volume of the peas and beads 10mL. The peas and beads were then removed from the cylinder and placed on another paper towel, they will make us respirometer 2

The graduated cylinder was refilled with 25mL of O. Then the amount of glass beads it takes to make a volume of 10mL was found using the displacement of water after the beads were placed in the cylinder. After the volume was found the beads were placed on a paper towel, they will be used in respirometer 3.

Steps 1-4 were repeated and the repeated germinating peas make up respirometer 4, the repeated non germinating peas and the glass beads make up respirometer 5, and the repeated glass beads made up respirometer 6.

Each respirometer consisted of one vial, one stopper and one pipette. After those were obtained an absorbent cotton ball was placed at the bottom of each vile and soaked with 1mL of 15% KOH. Next a non absorbent cotton wad was placed on top of the cotton ball.

Respirometer 1 and 4 consisted of a volume of 10mL germinating peas on top of the absorbent and non absorbent cotton. Respirometer 2 and 5 consisted of a volume of 10mL non germinating peas and glass beads on top of the absorbent and non absorbent cotton. Respirometer 3 and 6 consisted of a volume of 10mL glass beads on top of the absorbent and non absorbent cotton.

A sling was made out of a large piece of tape attached to the sides of the baths (large trays). The sling was to allow the pipettes to stay out of the water for the equilibrium period of 7 minutes. The respirometers 1, 2, and 3 were placed in the room temperature bath, and the respirometers 4, 5, and 6 were placed in the 10&deg;c bath.

After the equilibrium period of 7 min passed the respirometers in both baths were submerged entirely in their bath waters. The pipettes were moved so the labels could be read left to right 0-9, after this the water was never breached by human hands until the end of the 20 min testing period.

The respirometers were allowed to sit for 3 min to make sure they reached the equilibrium then the water in each pipette was recorded. Then for the next 20 minute in 5 minute intervals the water in each pipette was observed and recorded.

Table 1

Table 2

(Respirometer 6)

(Respirometer 5)

Germinating peas

(Respirometer 4)

Tem

(&deg;c)

Time

(min)

20&deg;

0

.82

.745

.815

5

.87

.69

.86

10

.88

.68

.89

15

.9

.67

.91

20

.91

.69

.92

Table 3

(Respirometer 3)

(Respirometer 2)

Germinating peas

(Respirometer 1)

Tem

(&deg;c)

Time

(min)

Diff.*

Diff*

Corrected

Diff.Δ

Diff.*

Corrected

Diff.Δ

20&deg;

0

.88

.89

.73

5

.88

0

.89

0

0

.53

.2

.2

10

.87

.01

.9

-.01

-.02

.41

.32

.31

15

.87

.01

.9

-.01

-.02

.33

.4

.39

20

.87

.01

.91

-.02

-.03

.22

.51

.5

Table 4

(Respirometer 6)

(Respirometer 5)

Germinating peas

(Respirometer 4)

Tem

(&deg;c)

Time

(min)

Diff.*

Diff*

Corrected

Diff.Δ

Diff.*

Corrected

Diff.Δ

10&deg;

0

.82

.745

.815

## -

5

.87

-.05

.69

-.045

.005

.86

.055

.105

10

.88

-.06

.68

-.075

-.015

.89

.065

.125

15

.9

-.08

.67

-.095

-.015

.91

.075

.155

20

.91

-.09

.69

-.105

-.015

.92

.055

.145

Graph 1

Graph 2

Graph 3

The bead group (respirometers 3 and 6) were the central group and their water in there pipettes should mot have changed, and since it did it suggest a change in temperature of the bath or pressure of the air. Since the T changed and the R is constant then the P would change (rule 4) and that is an explanation for the data. In the nongerminating peas (respirometer 2 and 5) and germinating peas (respirometer 1 and 4) rule 2 would apply. If the T and V remain constant then the P of the gas changes in direct proportion to the number of molecules of gas present.

The water in all the pipettes was at the end nearest to the peas and beads. The respirometers 1 and 4 (germinating peas) did soak up the most water and produce the gas. They also had the most gas at the end of the pipette. It was possible that the P could change also it was possible the T would change in the brief time between check ups of the thermostat.